Biohazard control and containment in oncogenic virus research

Publisher: U.S. Govt. Printing Office. in [Washington

Written in English
Published: Pages: 31 Downloads: 751
Share This


  • Laboratory infections -- Prevention,
  • Tumors -- Growth,
  • Oncogenic viruses,
  • Zoonoses -- Prevention

Edition Notes

Prepared in part by Biohazards Department, Dow Chemical Co., under contract PH43-65-1045 for the Biohazards Control and Containment Segment, National Cancer Institute

Statementedited by Alfred Hellman
ContributionsHellman, Alfred, National Cancer Institute (U.S.). Biohazards Control and Containment Segment
The Physical Object
Pagination31 p. :
Number of Pages31
ID Numbers
Open LibraryOL18138770M

Bio-related research activities may involve manipulation of microbial, animal or plant cells. limit the infectivity of vector to specific hosts and control the host-vector survival in the environment. Physical Containment (PC) The four biosafety levels corresponds to four risk groups. A lower risk group can be assigned a higher biosafety. containment measures required at containment level 2 (CL2) and 3 (CL3); and should be used in conjunction with the control measures specified in Schedule 3, COSHH. Further details on biosafety management in containment level 4 (CL4) laboratories can be found in the Principles, Design and Operation of Containment Level 4 Facilities. Your research does not appear to be covered by the Institutional Biosafety Committee. You do not need to submit this form. If you have questions, please contact the Biosafety Officer to discuss your research . The most frequent causes of cell culture contamination are poor techniques and housekeeping and inadequate sterility testing of supplies and culture media. The most common contaminants of cell cultures areMycoplasma, Torula sp., andPseudomonas sp. Routine procedures used in this laboratory to control or prevent accidental contamination include the use of filtered laminar air flow in transfer.

1. Represents a basic level of containment 2. Relies on standard microbiological practices 3. No special primary or secondary barriers other than a sink Example organisms: Bacillus subtilis-Naegleria gruberi-infectious canine hepatitis virus-exempt organisms under the NIH Recombinant DNA Guidelines. contamination from wild type virus can result in the presence of replication competent virus (RCV) in a population of replication deficient viral vectors. This policy outlines the biosafety levels and containment for commonly used vector systems. Research Oversight. the IBC for BSL-2 containment and sorting Cells exposed to microbial agents (e.g., viral, bacterial, fungal, protozoan, parasitic) with moderate increased personal hazard. (e.g. West Nile Virus) or Cells that have been genetically modified using viral methods with known oncogenic inserts AND Have been approved by EH&S and.   All Research involving the following materials shall require registration with the IBC. • All recombinant DNA (including research that is exempt from the NIH Guidelines) • Select agents and toxins* • Human and other primate pathogens assigned to biosafety level 3 or 4 • Oncogenic biological material *Final approval remains, under federal law, the purview of the Responsible Official or.

Foot and mouth virus escaped in from a veterinary lab in Surrey and in the SARS virus escaped from a high-containment lab in Beijing, infecting nine people before it was stopped. Show 0. See the WSU Bloodborne Infectious Diseases Exposure Control Plan for our written policy to comply with the MIOSHA Standard. Use of human materials in research. Research involving the use of human cell lines, tissue, blood, and/or other potentially infectious material is handled at Biosafety Level 2 (BSL-2). While this research does not require. AAV is usually classified as a Risk Group 1 (RG1) agent, but the appropriate biosafety level determination will depend on the nature of the gene that the vector carries. AAV constructs that do not encode an oncogenic or toxic transgene can be handled at BSL1. If helper virus is used during the - production of the AAV, BSL2 containment is required.

Biohazard control and containment in oncogenic virus research Download PDF EPUB FB2

Biohazard control and containment in oncogenic virus research. [Bethesda, Md.?]: U.S. Dept. of Health, Education, and Welfare, National Institutes of Health, U.S. Public Health Service ; Washington, D.C.: For sale by the Supt. of Docs., U.S.G.P.O., [] (OCoLC) Material Type: Government publication, National government publication.

Author(s): Hellman,Alfred; Dow Chemical Company. Biohazards Department; National Cancer Institute (U.S.). Biohazards Control and Containment Segment.

Title(s): Biohazard control and containment in oncogenic virus by Alfred Hellman. Country of Publication: United States Publisher: [Bethesda, Md., National Institutes of Health, ?]. The Hot Zone: A Terrifying True Story is a best-selling nonfiction thriller by Richard Preston about the origins and incidents involving viral hemorrhagic fevers, particularly ebolaviruses and marburgviruses.

The basis of the book was Preston's New Yorker article "Crisis in the Hot Zone". The filoviruses—including Ebola virus, Sudan virus, Marburg virus, and Ravn virus—are Author: Richard Preston. material is handled at a minimum of Biosafety Level - 2 (BSL-2). This includes the following: • Cell lines (primary and established) of human/primate origin.

• Cell lines derived from lymphoid or tumor tissue. • Cell lines exposed to or transformed by any oncogenic virus. Biosafety level 1 (BSL1) represents a minimal containment level which relies on standard microbiological practices.

Biosafety level 2 (BSL2) is for laboratories that handle emerging viruses Biohazard control and containment in oncogenic virus research book containment level 2 such as HIV, dengue virus, rabies virus, that are not generally transmitted by airborne by: 9.

This Biosafety Manual was prepared to provide laboratory and clinical investigators current information on biohazard control. The manual will be updated as our knowledge of the risks involved in. virus from the vector components, • the nature of the transgene insert (e.g., known oncogenes or genes with high oncogenic potential may merit special care) • the vector titer and the total amount of vector, • the inherent biological containment of the animal host, if relevant, •.

Centers for Disease Control and Prevention Charles B. Millard, PhD. Lieutenant Colonel, U.S. Army Director, Division of Biochemistry Walter Reed Army Institute of Research Shanna Nesby-O’Dell, DVM, MPH.

Chief, External Activities Program and WHO Collaborating Center for Biosafety and Training Office of Health and Safety.

Chapter 3 - Containment Levels and Containment Zones. Containment (or "biocontainment") refers to the combination of physical design parameters and operational practices that protects personnel, the immediate work environment, the community, and the external environment from exposure to potentially hazardous biological Canadian Biosafety Standard (CBS), 2 nd.

Biorisk management • Laboratory biosecurity guidance • September ii Abbreviations BSL3 Containment laboratory – Biosafety Level 3 BSL4 Maximum containment laboratory – Biosafety Level 4 FAO Food and Agriculture Organization of the United Nations GMO Genetically modified organism LBM3 Laboratory biosafety manual, third edition, LBG Biorisk management: laboratory.

BIOHAZARD CABINET CONTAINMENT EQUIPMENT. Control of microbiological containment in the laboratory can be achieved by proper selection and use of ventilated cabinet containment equipment. It is generally accepted that potentially hazardous materials should be controlled at the source to protect personnel, product, and the environment.

National Research Council (US) Subcommittee on Amphibian Standards. Amphibians: Guidelines for the breeding, care, and management of laboratory animals.

Comparative studies of amphibian cytoplasmic virus strains isolated from the leopard frog, bullfrog, and newt, p– Biohazard control and containment in oncogenic virus research. If any cell or tissue cultures are known or suspected to contain a specific pathogen or oncogenic virus, appropriate biosafety practices for handling that virus shall be used when working with the cell or tissue culture.

BSL-1 practices and containment may be used for cell lines that meet all of. Both of their deaths involved research in biosafety level 2 labs, where pathogens are considered to be less dangerous than those worked with in high-containment labs.

Available through Research Safety: Biohazard Bags: Label with Biohazard Waste Labels and collect biohazard waste for disposal in Research Safety-provided collection bins. Use secondary containment while filling the bag and to transport all waste.

Do not put anything in the bag that may puncture it such as pipets or other rigid materials. How to use this tool How to use this tool. Stanford University follows the categorizing of infectious agents into levels as described in Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th edition, written and published by the Centers for Disease Control (CDC) and NIH.

The descriptions of biosafety levels (BSL) 1 – 4 parallel those in the NIH Guidelines for research. The biohazard symbol was developed in by Charles Baldwin, an environmental-health engineer working for the Dow Chemical Company on the containment products.

It is used in the labeling of biological materials that carry a significant health risk, including. The University of Pittsburgh IBC will apply allof the following criteria for determining the appropriate biosafety containment and handling of AAV/rAAV: • Propagation with or without helper virus, including the use of adenovirus • Presence of transgenes encoding oncogenes or toxins • Propagation in insect cell lines versus human cell lines • Verification of the purification techniques and quality control assays used when propagation of virus.

consequences of such infection. The levels of containment usually required for work with such agents are determined by their categorisation (Containment Level [CL] 3 is required for Hazard Group 3 pathogens) and these reflect the increasing levels of health risk to those involved in (or who could be affected by) such work.

Contact the Office of Research Assurances for information. Human & non-human primate cells. Including all cell lines. Tissue, blood, and potentially infectious fluids.

For details, see section XVII of the IBC Manual (pdf). Animals or vectors known or suspected to be reservoirs of Risk Group 2 or 3 infectious agents requiring BSL 2 or 3 containment.

Centers for Disease Control. Agent summary statement for human immunodeficiency virus and report on laboratory acquired infection with human immunodeficiency virus. MMWR S-4. Embedded in the sprawling campus of the National Institutes of Health (NIH) in Bethesda, Md., is a small but mighty high-level containment facility known as the Special Clinical Studies Unit.

research infectious diseases; to prevent escape of infec-tious agents, to minimize staff member’s and other peo-ple’s contact with infectious agents, both inside and out-side the containment zone, and to prevent the introduc-tion of infectious agents into nature.

Some biosafety guidelines take a. New display cases have been installed around campus. Read a comic book about Joseph Goldberger’s work in pellagra in the early 20 th century at the Building 1, 3 rd floor case.

Be amazed at the variety of Clinical Center patches near the Hospitality Desk on the 1 st floor of the Clinical Center. Think about the social context of coloring books from the Clinical Center by its 2 nd floor.

Replication Compentent: No - needs helper virus (CMV, adenovirus, herpesvirus, vaccinia) RCV Testing: If helper virus is adenovirus, presence of RCV after heat inactivation (56C for 15min) Recombination: Possible. Use of cells with E1 deletion can cause systematic rescue Transgene: Evaluate if oncogenic or biotoxic Ecotropic/Xenotropic.

Biosafety Level 2 (BSL-2) protections apply to work with pathogens or infectious organisms associated with human diseases. These organisms pose a moderate health hazard. Centers for Disease Control and Prevention (CDC) guidelines (pdf) require BSL-2 containment for work with HEK cell lines.

Unfortunately, this book can't be printed from the OpenBook. If you need to print pages from this book, we recommend downloading it as a PDF.

Visit to get more information about this book, to buy it in print, or to download it as a free PDF. CDC/NIH: Biosafety in Microbiological and Biomedical Laboratories, 3 rd edition, NIH - Working Safely with HIV in the Research Laboratory Biosafety Level, 2/3, CDC Guidelines for protection against Viral Hepatitis and Hepatitis B prevention.

NCI Biological Safety Manual for Research Involving Oncogenic Viruses. The Institutional Biosafety Committee (IBC) is a University-wide Committee responsible for reviewing and approving recombinant DNA research and biohazard projects.

The committee sets containment levels in accordance with the National Institutes of Health (NIH) Guidelines and the Center for Disease Control and Prevention. Printed and spiral bound copies of the manual are available for pickup upon request, please call () for more information.

This revision of the Biosafety Manual was prepared under the auspices of the Administrative Panel on Biosafety (APB) by the Office of Environmental Health and Safety (EH&S) after careful review of pertinent federal and state government regulatory.

A. Definition -- Biological safety or “biosafety” is the application of knowledge, techniques and equipment to prevent personal, laboratory and environmental exposure to potentially infectious agents or biohazards. Biosafety defines the containment conditions under which infectious agents can be safely manipulated.Research and Development Office.

Transgenes express an oncogenic protein or toxin 2. Helper virus of human origin is used to generate AAV/rAAV *All investigators who receive IBC approval for specified rAAV at a downgraded biosafety containment level (BSL-1/ABSL-1) are required to keep the AAV quality control data for the.

According to the standard, HVAC system of BSL-3 large-scale production facility must be able to control absolute negative pressure in the containment area at any time; meanwhile, ensure that the system can meet basic biosafety requirements when working conditions change (often with the highest biosafety risk), including system startup.